What are the 3Rs?


Methods which permit a given purpose to be achieved without conducting experiments or other scientific procedures on animals.

Replacement methods can represent full or partial replacement methodologies, based on i) use of non-animal approaches including testing strategy, human data, in vitro methods, in silico and computational methods, physicochemical properties and non-testing data, and/or on ii) use of animals that, based on current scientific evidence, are not considered capable of experiencing suffering such as some invertebrates and immature forms of vertebrates. It also includes the use of primary cells, tissues and organs.


Methods for obtaining comparable levels of information from the use of fewer animals in scientific procedures, or for obtaining more information from the same number of animals.

Reduction methods include careful design and analysis of animal-based experiments so that fewer animals can be used, such as optimization of breeding programmes, experimental designs, statistical analyses, as well as  sharing of animals and animal material (organs, tissues, cells), and, if appropriate longitudinal instead of cross-sectional measurements, and measures to reduce unexplained variation in the data.


Methods which alleviate or minimise potential pain, suffering and distress, and which enhance animal well-being.

Refinement applies to all aspects of animal care and use, including housing conditions, handling methods, anaesthesia and analgesia, habituation to procedures, execution of procedures, monitoring of health and well-being, humane endpoints, and euthanasia. Refinement also includes the development of better (i.e. more accurate, reliable, sensitive) tools to assess suffering and well-being.