Japanese researchers create a vascularised 3D model of human oral mucosa

A team of researchers from Japan used a layer-by-layer cell coating technique to develop a 3D model to study human oral mucosa in the lab. According to the authors, this new model is a promising alternative to animal testing and brings new possibilities to the field of regenerative medicine. In the study published in the journal Tissue Engineering the authors describe how they created a lamina propria equivalent – made up of human oral mucosa fibroblasts in extracellular matrix. They seeded keratinised or non-keratinised epithelial cells onto this construct and in some models included human umbilical vein endothelial cells to create blood vessels.  The authors showed that their models displayed appropriate features of native human tissue. More info: https://www.liebertpub.com/doi/10.1089/ten.TEC.2018.0337