Transparent, standardised methods are essential for advancing human-relevant research and the 3Rs. This collection of step-by-step videos and standard operating procedures (SOPs) captures best practices refined through rigorous optimisation during the project, supporting reproducibility and reliable transfer of a human in vitro blood–brain barrier model.

Developed by Valerio Taggi (Biopharmacy Group, Department of Pharmaceutical Sciences, University of Basel) as an outcome of his funded doctoral project Establishment and validation of a human in vitro model to investigate uptake transport at the Blood-Brain Barrier, these openly accessible resources provide clear guidance on fundamental cell culture techniques. By reducing methodological variability and lowering technical barriers, they support wider adoption of this alternative model and foster collaboration across research groups.

Below you can find instructional videos for the key technical steps used in the hBMEC blood-brain barrier model:

Below you can find the SOPs for the various techniques used in the hBMEC blood-brain barrier model: