Refinement of anesthesia in neonatal mice

Keywords: neonatal mice, anesthesia, hypothermia, isoflurane, fMRI, c-fos and apoptosis
Budget: CHF 24'000.-

Aim

• The choice of anesthesia for neonatal mice lacks a consensus and directly impacts animal welfare and research outcomes.

• The methods used until now to assess animal welfare do not provide a complete understanding of potential pain or discomfort experienced during anesthesia.

• We aim to compare hypothermia and isoflurane anesthesia in neonates to better understand their impact on animal welfare.

• Our goal is to find the best method to reduce the stress and/or pain experienced by neonates during anesthesia and therefore the severity degree of procedures.

• Determine the most humane anesthesia method for neonates to refine animal experiments and improve well-being.